In an attempt, to uncover the molecular patterns of the brain regions, explaining how stress-related disorders have a role, and the effective tools that can make an effective diagnostics possible; an investigative study was carried out on the molecular changes that occur in the brains of individuals suffering from post-traumatic stress disorder (PTSD) and major depressive disorder (MDD). The researchers also hope that this would advance treatments for the patients. Previous studies have demonstrated that hormonal, immune, methylomic and transcriptomic factors have the potential to contribute but there wasn’t much studies conducted on the post-mortem brain tissues.
Data was analysed from 231 PTSD, MDD and neurotypical cohort subjects, including 114 individuals from the replication cohorts, to look for differences in the three brain regions-the medial prefrontal cortex, hippocampal dentate gyrus, and the central nucleus of amygdala. More than 50,000 Biobank participants had taken part, in order for them to study the cell-type specfic patterns and evaluate blood based proteins. Single nucleus RNA sequencing of the 118 PFC (pre-frontal cortex) samples was performed.
They compared the genes of PTSD and MDD to the genome wide association studies (GWAS), to determine the risk of both the diseases. Despite PTSD and MDD both having an altered gene expression, and exons in the mPFC, the epigenetic changes that occurred in the localisation differed in each. They want to figure out the reason, why and how some people develop PTSD and depression, and others do not. Dr Charles B. Nemeroff continues on stating that they have discovered molecular differences, particularly in the prefrontal cortex of these patients.
Daskalakis added that their findings can support the development of brain blood markers. Ressler is hopeful that these biomarkers could help in overcoming the challenges that arise when obtaining brain biopsies to advance treatments for patients. Their research work was possible thanks to the donations of the brain tissue and matter made possible by the patients who died from these pychiatric conditions to the Lieber Institute of Brain Repository. The results of this study were published in the journal Science.
The genetic components of this study were created on a study published in April, in the journal of Nature Genetics.
Nivea Vaz
Manipal College of Medical Sciences, Pokhara







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